DIAG4ZOO

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DIAG4ZOO has been retained by a French research institute to verify by qPCR the presence of Nodavirus in sea bass farms. This type of analysis comes in complement of the Nodavirus kit proposed by the company and used by other French teams present in overseas

Montpellier, October 26th 2020

The Nodavirus, a RNA virus, causes a disease of the central nervous system and the retina of fish: the viral nervous necrosis (VNN), also called the viral encephalopathy and retinopathy (VER). This serious disease affects a large number of marine fish and causes significant losses in the following species: sea bass, turbot, umbra, lean, bream, sole, halibut, grouper, and some aquarium fishes.

Nodavirus infection causes nervous disorders in an increasing number of marine species: larvae and juveniles are the most susceptible to infection with a high mortality rate.

DIAG4ZOO acquired for many years an expertise in the realization of analyses by qPCR to detect the presence of the Nodavirus in samples of fins of various fish populating different ecosystems (platax and ombrine in Tahiti, grouper and sea bass of the Mediterranean, sea bass of the Atlantic coasts), but also other marine animals, such as the holothurian (sea cucumber) lagoon.

On the basis of this expertise, a French research institute asked DIAG4ZOO to carry out analyses by qPCR to verify the presence of this Nodavirus in the sea bass farms under surveillance by this institute.

This qPCR analysis service confirms the technical and scientific expertise of the company’s team and the quality of its engineering to meet its customers’ needs as closely as possible.

DIAG4ZOO also markets a Nodavirus detection kit. This test is based on the enzymatic amplification of a specific nodavirus sequence by PCR. The amplified products are then deposited (hybridized) on membranes (mini-array) ready to use. In the case of presence of the nodavirus, one notices visually (with the naked eye) a colorimetric revelation of the amplified products deposited on the membrane. This kit is used by a research institute present in overseas to check the presence of the nodavirus in ponds of breeding of platax.